{"id":8801,"date":"2024-08-06T10:34:54","date_gmt":"2024-08-06T08:34:54","guid":{"rendered":"https:\/\/www.mio.osupytheas.fr\/?page_id=8801"},"modified":"2024-09-19T09:59:04","modified_gmt":"2024-09-19T07:59:04","slug":"spectrom","status":"publish","type":"page","link":"https:\/\/www.mio.osupytheas.fr\/en\/research\/technical-facilities\/spectrom\/","title":{"rendered":"SPECTROM"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"8801\" class=\"elementor elementor-8801\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2ce2260 e-flex e-con-boxed e-con e-parent\" data-id=\"2ce2260\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-478385a e-flex e-con-boxed e-con e-child\" data-id=\"478385a\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-b3ad13b elementor-widget__width-initial elementor-widget elementor-widget-theme-post-title elementor-page-title elementor-widget-heading\" data-id=\"b3ad13b\" data-element_type=\"widget\" data-widget_type=\"theme-post-title.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">SPECTROM<\/h1>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1a6cbdd elementor-widget__width-initial breadcrumb elementor-widget elementor-widget-shortcode\" data-id=\"1a6cbdd\" data-element_type=\"widget\" data-widget_type=\"shortcode.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-shortcode\"> <div class=\"breadcrumb-container theme1\">\n\n            <ol>\n                            <\/ol>\n        <\/div>    <script type=\"application\/ld+json\">{\n    \"@context\": \"http:\\\/\\\/schema.org\",\n    \"@type\": \"BreadcrumbList\",\n    \"itemListElement\": []\n}<\/script>\n    <style type=\"text\/css\">\n        .breadcrumb-container {\n            font-size: 13px;\n        }\n\n        .breadcrumb-container ul {\n            margin: 0;\n            padding: 0;\n        }\n\n        .breadcrumb-container li {\n            box-sizing: unset;\n            display: inline-block;\n            margin: 0;\n            padding: 0;\n        }\n\n        .breadcrumb-container li a {\n            box-sizing: unset;\n            padding: 0 10px;\n        }\n\n        .breadcrumb-container {\n            font-size: 16px !important;\n                    }\n\n        .breadcrumb-container li a {\n            color: #f0f0f0 !important;\n            font-size: 16px !important;\n            line-height: 16px !important;\n                    }\n\n        .breadcrumb-container li .separator {\n            color: #f0f0f0 !important;\n            font-size: 16px !important;\n                    }\n\n        .breadcrumb-container li:last-child .separator {\n            display: none;\n        }\n    <\/style>\n    <style type=\"text\/css\">\n        .breadcrumb-container.theme1 li {\n            margin: 0;\n            padding: 0;\n        }\n        .breadcrumb-container.theme1 a {\n            background: ;\n            display: inline-block;\n            margin: 0 5px;\n            padding: 5px 10px;\n            text-decoration: none;\n        }\n    <\/style>\n        <style type=\"text\/css\">\n        .breadcrumb-container{}\r\n.breadcrumb-container ul{}\r\n.breadcrumb-container li{}\r\n.breadcrumb-container a{\r\n\tfont-weight:300;\r\n\tline-height:23px !important;\r\n}\r\n.breadcrumb-container .separator{}\r\n    <\/style>\n    <script>\n            <\/script>\n <\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-584baca e-con-full e-flex e-con e-parent\" data-id=\"584baca\" data-element_type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-17303fe e-con-full e-flex e-con e-child\" data-id=\"17303fe\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2849cf0 elementor-widget elementor-widget-heading\" data-id=\"2849cf0\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Spectroscopy, Optics and Imaging - Carbon, Nitrogen<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a3bd473 elementor-widget elementor-widget-text-editor\" data-id=\"a3bd473\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div>Le P\u00f4le Spectroscopie, Optique et Imagerie &#8211; Carbone, Azote rassemble les comp\u00e9tences de l&rsquo;\u00e9quipe CEM dans l&rsquo;analyse par spectroscopie de fluorescence (continue et r\u00e9solue en temps) et d&rsquo;absorbance, appliqu\u00e9es notamment \u00e0 la caract\u00e9risation de la mati\u00e8re organique (MO) ou la d\u00e9tection de compos\u00e9s fluorescents, en laboratoire mais \u00e9galement on-site et in-situ. En compl\u00e9ment \u00e0 cette caract\u00e9risation de la MO, le p\u00f4le propose aussi des analyses de carbone et d\u2019azote total dans les compartiments liquide et solide.<\/div><div>\u00a0<\/div><div>Le P\u00f4le a acquis r\u00e9cemment un microscope Confocal\/biphoton \u00e0 balayage laser qui permet d&rsquo;obtenir des images de grande r\u00e9solution. Des coupes \u00ab\u00a0optiques\u00a0\u00bb d&rsquo;une \u00e9paisseur d&rsquo;environ 0.4\u00b5m sont obtenues \u00e0 diff\u00e9rents niveaux de l&rsquo;\u00e9chantillon qui permettent une reconstruction 3D de l\u2019\u00e9chantillon.<\/div><div>\u00a0<\/div><div>This microscope is also capable of providing fluorescence lifetime images (FLIM) from a few hundred picoseconds down to microseconds. A typical application of FLIM is FLIM - FRET (F\u00f6rster Resonance Energy Transfer). FRET is a well-established technique for studying molecular interactions. The cluster will develop these F-techniques to study complexations between organic matter and emerging pollutants.<\/div><div>\u00a0<\/div><div>Complementary techniques are used to determine concentrations of dissolved organic and inorganic carbon (DOC and DIC) using a TOC-V analyser and total nitrogen (TN) using the appropriate module. A CHNS will provide an elemental analysis of the solid matrix (soil, sediment, etc.).<\/div>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-df62c6a elementor-widget elementor-widget-button\" data-id=\"df62c6a\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/www.mio.osupytheas.fr\/en\/research\/research-teams\/coastal-physical-oceanography-team-oplc\/improving-the-representation-of-ocean-dynamics-by-understanding-multi-scale-processes-2\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">More information<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-beccd5c e-con-full e-flex e-con e-child\" data-id=\"beccd5c\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-47bdfd5 e-con-full e-flex e-con e-parent\" data-id=\"47bdfd5\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t<div class=\"elementor-element elementor-element-4571c06 e-flex e-con-boxed e-con e-child\" data-id=\"4571c06\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-8be6031 e-con-full e-flex e-con e-child\" data-id=\"8be6031\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5742e74 elementor-widget-mobile__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"5742e74\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h3 class=\"rtejustify\"><span style=\"color: #003366;\"><strong>How it works<\/strong><\/span><\/h3><p class=\"rtejustify\">SPECTROM is located on the premises of the Institut M\u00e9diterran\u00e9en d'Oc\u00e9anologie (MIO), at the University of Toulon on 1<sup>er<\/sup> floor and 2<sup>th<\/sup> building R, Campus La Garde.<\/p><p class=\"rtejustify\">The referents are :<\/p><p class=\"rtejustify\"><strong><a href=\"\/en\/directory\/christophe-le-poupon\/\">Christophe LE POUPON<\/a><\/strong><strong>\u00a0\u00a0\u00a0 <\/strong>Tel: 04 94 14 25 02 - e-mail: <a href=\"mailto:lepoupon@univ-tln.fr\">lepoupon@univ-tln.fr<\/a><\/p><p class=\"rtejustify\"><strong><a href=\"\/en\/directory\/houssam-hajjoul\/\">Houssam HAJJOUL\u00a0<\/a><\/strong>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0 \u00a0 \u00a0 Tel: 04 94 14 27 94 - e-mail: <a href=\"mailto:hajjoul@univ-tln.fr\">hajjoul@univ-tln.fr<\/a><\/p><h3 class=\"rtejustify\"><span style=\"color: #003366;\"><strong>Access conditions <\/strong><\/span><\/h3><p class=\"rtejustify\">The Centre is accessible to all <strong>MIO<\/strong> and the scientific community: academics, industrialists and clinicians with study needs in UV-Visible spectroscopy (continuous and time-resolved fluorescence, fluorescence quenching, in-situ monitoring, etc.) and confocal microscopy (biphoton, 3D imaging, FLIM, FRET, FRAP, etc.).<\/p><p class=\"rtejustify\">To gain access to the self-service equipment, new users must undergo training on the equipment required, provided by the Hub's staff. Without this training, no novice user will be allowed to use the equipment. Access to the laser rooms is reserved for people with laser level I.<\/p><p class=\"rtejustify\">The Centre also offers simple services based on a research project or complete services from A to Z, including data analysis and results. In this case, the service is provided on the basis of a quotation after your request has been studied.<\/p><p class=\"rtejustify\">Users must provide all information relating to the possible risks for handlers. The cost of analysis is given in the summary table.<\/p><h3 class=\"rtejustify\"><span style=\"color: #003366;\"><strong>Use of equipment<\/strong><\/span><\/h3><p class=\"rtejustify\">Les utilisateurs autoris\u00e9s \u00e0 l\u2019acc\u00e8s libre s&rsquo;engagent \u00e0 remplir syst\u00e9matiquement le \u00ab cahier du suivi des utilisateurs \u00bb mis \u00e0 leur disposition et de signaler sur le cahier et sur GRR les probl\u00e8mes rencontr\u00e9s lors de l\u2019utilisation. En cas de dysfonctionnement, ils doivent contacter le responsable de l\u2019\u00e9quipement. Chaque utilisateur est responsable de l\u2019\u00e9tat de propret\u00e9 dans lequel il laisse l\u2019appareil en fin de s\u00e9ance.<\/p><h3 class=\"rtejustify\"><span style=\"color: #003366;\"><strong>Reservations<\/strong><\/span><\/h3><p class=\"rtejustify\">Equipment can only be booked via the website:<br \/>Intranet : <a href=\"https:\/\/grr.univ-tln.fr\/\" target=\"_blank\" rel=\"noopener\">https:\/\/grr.univ-tln.fr\/ <\/a>or by PSOI-CN staff after validation of the analysis request. Users are notified by e-mail when a device is unavailable.<\/p><h3 class=\"rtejustify\"><span style=\"color: #003366;\"><strong>Services<\/strong><\/span><\/h3><p class=\"rtejustify\">For a service or collaboration, users (applicants) must request an appointment by e-mail or telephone with the Centre's managers in order to establish the costs and, if necessary, an estimate.<\/p><p class=\"rtejustify\">The P\u00f4le can provide the following services:<\/p><p class=\"rteindent1 rtejustify\"><strong>A.<\/strong> A consultancy business to study the fluorescence of organic matter<br \/><strong>B. <\/strong>Support during acquisitions on different instruments and during analysis on PARAFAC or deconvolution on MATLAB<br \/><strong>C.<\/strong> Theoretical and practical training :<\/p><p class=\"rteindent2 rtejustify\">- Fluorescence spectroscopy \"source separation and time resolution - from photon to source\" (40H)<br \/>- Laser risks and prevention \"Laser risks and prevention awareness training for anyone working with laser sources\" (4H)<\/p><h4 class=\"rtejustify\"><span style=\"color: #003366;\"><strong>Fluorescence spectroscopy analysis<\/strong><\/span><\/h4><ul class=\"rteindent1\"><li class=\"rtejustify\">\u00a02D Fluorescence Spectrum Measurement<\/li><li class=\"rtejustify\">\u00a03D Fluorescence Spectrum Measurement<\/li><li class=\"rtejustify\">\u00a0Fluorescence quenching<\/li><li class=\"rtejustify\">\u00a0Fluorescence Emission Excitation Matrix (FEEM)<\/li><li class=\"rtejustify\">\u00a0Time-to-live measurements (TRLF) on liquid and solid samples with lifetimes from nanoseconds to microseconds.<\/li><li class=\"rtejustify\">\u00a0PARAFAC breakdown of the ProgMEEF and ProgTIME programmes developed in the laboratory by Roland Redon<\/li><li class=\"rtejustify\">\u00a0Mesure de l\u2019absorption dans le domaine de longueurs d&rsquo;onde 200-800 nm par Spectroscopie UV-Visible.<\/li><\/ul><h4 class=\"rtejustify\"><span style=\"color: #003366;\"><strong>Confocal UV, Visible and Biphotonic imaging analyses <\/strong><\/span><\/h4><ul class=\"rteindent1\"><li class=\"rtejustify\">\u00a03D imaging.<\/li><li class=\"rtejustify\">\u00a0Spectral imaging.<\/li><li class=\"rtejustify\">\u00a0Fluorescence redistribution after photobleaching (FRAP, FLIP)<\/li><li class=\"rtejustify\">\u00a0Fluorescence Lifetime (FLIM)<\/li><li class=\"rtejustify\">\u00a0Le transfert r\u00e9sonnant d&rsquo;\u00e9nergie de type F\u00f6rster par FLIM<\/li><\/ul><h4 class=\"rtejustify\"><span style=\"color: #003366;\"><strong>Equipment <\/strong><\/span><\/h4><ul class=\"rteindent1\"><li class=\"rtejustify\">\u00a0HITACHI F-7000<\/li><li class=\"rtejustify\">\u00a0HITACHI F-4500 with sample holder for automatic measurement of 16 samples<\/li><li class=\"rtejustify\">\u00a0SHIMADZU UV-1800<\/li><li class=\"rtejustify\">\u00a0Laser Spectra-Physics Quanta-Ray INDI Nd:YAG Wavelengths: 1064 nm, 532 nm, 355 nm and 266 nm -Pulse width: 3-5 ns - Frequency: 20 Hz<\/li><li class=\"rtejustify\">\u00a0EKSPLA picosecond Nd:YAG laser Wavelengths: 1064 nm, 532 nm, 355 nm and 266 nm - Pulse width: 20 ps - Frequency: 20 Hz<\/li><li class=\"rtejustify\">\u00a0Zeiss upright microscope LSM 880 Laser Visible 458, 488, 514, 561, 633 nm - LASER Ti:Sapphire Chameleon Ultra II from 680 to 1080 nm - HARMONIXX UV PULSED LINE 340 to 540 nm - FLIM-Becker system for lifetime measurement - Chamber with temperature regulation\/gas injection<\/li><li class=\"rtejustify\">TOC-V analyser - SHIMADZU<\/li><li class=\"rtejustify\">Total nitrogen analyser, NT. TNM-1 module - SHIMADZU<\/li><li class=\"rtejustify\">Elemental analyser CHNS, FLASH 2000 - ThermoScientific<\/li><\/ul><p class=\"rtejustify\">Download the price list in pdf : <a href=\"https:\/\/nuage.osupytheas.fr\/s\/ziNHEQrPe4BCYcM\" target=\"_blank\" rel=\"noopener\">ICI<\/a> <em>(clickable link)<\/em><\/p><p class=\"rtejustify\">Fiche de demande d&rsquo;analyses : <a href=\"https:\/\/nuage.osupytheas.fr\/s\/FDGs7BdnLMH9Ysw\" target=\"_blank\" rel=\"noopener\">ICI<\/a> <em>(clickable link)<\/em><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-0d694e1 e-con-full e-flex e-con e-parent\" data-id=\"0d694e1\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t<div class=\"elementor-element elementor-element-a4d9462 e-flex e-con-boxed e-con e-child\" data-id=\"a4d9462\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-acd5740 elementor-widget elementor-widget-heading\" data-id=\"acd5740\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Visit SPECTROM in pictures<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-6113b4f e-con-full e-flex e-con e-parent\" data-id=\"6113b4f\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t<div class=\"elementor-element elementor-element-1b33e73 e-con-full e-flex e-con e-child\" data-id=\"1b33e73\" data-element_type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-ea44992 e-con-full e-flex e-con e-child\" data-id=\"ea44992\" 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