{"id":8801,"date":"2024-08-06T10:34:54","date_gmt":"2024-08-06T08:34:54","guid":{"rendered":"https:\/\/www.mio.osupytheas.fr\/?page_id=8801"},"modified":"2024-12-12T20:05:06","modified_gmt":"2024-12-12T19:05:06","slug":"spectrom","status":"publish","type":"page","link":"https:\/\/www.mio.osupytheas.fr\/en\/research\/technical-facilities\/spectrom\/","title":{"rendered":"SPECTROM"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"8801\" class=\"elementor elementor-8801\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2ce2260 e-flex e-con-boxed e-con e-parent\" data-id=\"2ce2260\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-478385a e-flex e-con-boxed e-con e-child\" data-id=\"478385a\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-b3ad13b elementor-widget__width-initial elementor-widget elementor-widget-theme-post-title elementor-page-title elementor-widget-heading\" data-id=\"b3ad13b\" data-element_type=\"widget\" data-widget_type=\"theme-post-title.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">SPECTROM<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1a6cbdd elementor-widget__width-initial breadcrumb elementor-widget elementor-widget-shortcode\" data-id=\"1a6cbdd\" data-element_type=\"widget\" data-widget_type=\"shortcode.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-shortcode\"> <div class=\"breadcrumb-container theme1\">\n\n            <ol>\n                            <\/ol>\n        <\/div>    <script type=\"application\/ld+json\">{\n    \"@context\": \"http:\\\/\\\/schema.org\",\n    \"@type\": \"BreadcrumbList\",\n    \"itemListElement\": []\n}<\/script>\n    <style type=\"text\/css\">\n        .breadcrumb-container {\n            font-size: 13px;\n        }\n\n        .breadcrumb-container ul {\n            margin: 0;\n            padding: 0;\n        }\n\n        .breadcrumb-container li {\n            box-sizing: unset;\n            display: inline-block;\n            margin: 0;\n            padding: 0;\n        }\n\n        .breadcrumb-container li a {\n            box-sizing: unset;\n            padding: 0 10px;\n        }\n\n        .breadcrumb-container {\n            font-size: 16px !important;\n                    }\n\n        .breadcrumb-container li a {\n            color: #f0f0f0 !important;\n            font-size: 16px !important;\n            line-height: 16px !important;\n                    }\n\n        .breadcrumb-container li .separator {\n            color: #f0f0f0 !important;\n            font-size: 16px !important;\n                    }\n\n        .breadcrumb-container li:last-child .separator {\n            display: none;\n        }\n    <\/style>\n    <style type=\"text\/css\">\n        .breadcrumb-container.theme1 li {\n            margin: 0;\n            padding: 0;\n        }\n        .breadcrumb-container.theme1 a {\n            background: ;\n            display: inline-block;\n            margin: 0 5px;\n            padding: 5px 10px;\n            text-decoration: none;\n        }\n    <\/style>\n        <style type=\"text\/css\">\n        .breadcrumb-container{}\r\n.breadcrumb-container ul{}\r\n.breadcrumb-container li{}\r\n.breadcrumb-container a{\r\n\tfont-weight:300;\r\n\tline-height:23px !important;\r\n}\r\n.breadcrumb-container .separator{}\r\n    <\/style>\n    <script>\n            <\/script>\n <\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-bcd19ca e-flex e-con-boxed e-con e-parent\" data-id=\"bcd19ca\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-427dfd0 elementor-widget elementor-widget-text-editor\" data-id=\"427dfd0\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: justify;\">\u00a0<\/p><p style=\"text-align: justify;\">Le <strong>Spectroscopy, Optics and Imaging Division - Carbon, Nitrogen<\/strong> brings together the CEM team's expertise in fluorescence spectroscopy (continuous and time-resolved) and absorbance analysis, applied in particular to the characterisation of organic matter (OM) or the detection of fluorescent compounds, in the laboratory as well as on-site and in-situ.<\/p><p style=\"text-align: justify;\">\u00a0In addition to this characterisation of OM, the centre also offers analyses of carbon and total nitrogen in the liquid and solid compartments.<\/p><p style=\"text-align: justify;\">The cluster recently acquired a laser scanning confocal\/biphoton microscope, which can be used to obtain high-resolution images. Optical\" sections with a thickness of around 0.4\u00b5m are obtained at different levels of the sample, enabling 3D reconstruction of the sample.<\/p><p style=\"text-align: justify;\">This microscope is also capable of providing fluorescence lifetime images (FLIM) from a few hundred picoseconds down to microseconds. A typical application of FLIM is FLIM - FRET (F\u00f6rster Resonance Energy Transfer). FRET is a well-established technique for studying molecular interactions. The cluster will develop these F-techniques to study complexations between organic matter and emerging pollutants.<\/p><p style=\"text-align: justify;\">Complementary techniques are used to determine concentrations of dissolved organic and inorganic carbon (DOC and DIC) using a TOC-V analyser and total nitrogen (TN) using the appropriate module. A CHNS will provide an elemental analysis of the solid matrix (soil, sediment, etc.).<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-75d8218 e-con-full e-flex e-con e-parent\" data-id=\"75d8218\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-6c48df3 elementor-widget elementor-widget-image\" data-id=\"6c48df3\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"720\" height=\"479\" src=\"https:\/\/www.mio.osupytheas.fr\/wp-content\/uploads\/2024\/12\/confocal-e1734030092666.png\" class=\"attachment-large size-large wp-image-17767\" alt=\"\" srcset=\"https:\/\/www.mio.osupytheas.fr\/wp-content\/uploads\/2024\/12\/confocal-e1734030092666.png 720w, https:\/\/www.mio.osupytheas.fr\/wp-content\/uploads\/2024\/12\/confocal-e1734030092666-300x200.png 300w, https:\/\/www.mio.osupytheas.fr\/wp-content\/uploads\/2024\/12\/confocal-e1734030092666-18x12.png 18w\" sizes=\"(max-width: 720px) 100vw, 720px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-47bdfd5 e-flex e-con-boxed e-con e-parent\" data-id=\"47bdfd5\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-8f6cd99 elementor-widget elementor-widget-text-editor\" data-id=\"8f6cd99\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4 class=\"rtejustify\" style=\"text-align: justify;\"><span style=\"color: #003366;\"><strong>How it works<\/strong><\/span><\/h4><p class=\"rtejustify\" style=\"text-align: justify;\"><strong>SPECTROM<\/strong> is located on the premises of the Institut M\u00e9diterran\u00e9en d'Oc\u00e9anologie (MIO), at the University of Toulon on 1<sup>er<\/sup> floor and 2<sup>th<\/sup> building R, Campus La Garde.<\/p><p class=\"rtejustify\" style=\"text-align: justify;\">The referents are :<\/p><p class=\"rtejustify\" style=\"text-align: justify;\"><a href=\"\/en\/directory\/christophe-le-poupon\/\">Christophe Le Poupon<\/a><strong> &#8211; <\/strong>Tel : 04 94 14 25 02 - mail : <a href=\"mailto:lepoupon@univ-tln.fr\">lepoupon@univ-tln.fr<\/a><\/p><p class=\"rtejustify\" style=\"text-align: justify;\"><a href=\"\/en\/directory\/houssam-hajjoul\/\">Houssam Hajjoul<\/a> <strong>&#8211;<\/strong> Tel : 04 94 14 27 94 - mail : <a href=\"mailto:hajjoul@univ-tln.fr\">hajjoul@univ-tln.fr<\/a><\/p><h4>\u00a0<\/h4><h4 class=\"rtejustify\" style=\"text-align: justify;\"><span style=\"color: #003366;\"><strong>Access conditions <\/strong><\/span><\/h4><p class=\"rtejustify\" style=\"text-align: justify;\">The Centre is accessible to all <strong>MIO<\/strong> and the scientific community: academics, industrialists and clinicians with study needs in UV-Visible spectroscopy (continuous and time-resolved fluorescence, fluorescence quenching, in-situ monitoring, etc.) and confocal microscopy (biphoton, 3D imaging, FLIM, FRET, FRAP, etc.).<\/p><p class=\"rtejustify\" style=\"text-align: justify;\">To gain access to the self-service equipment, new users must undergo training on the equipment required, provided by the Hub's staff. Without this training, no novice user will be allowed to use the equipment. Access to the laser rooms is reserved for people with laser level I.<\/p><p class=\"rtejustify\" style=\"text-align: justify;\">The Centre also offers simple services based on a research project or complete services from A to Z, including data analysis and results. In this case, the service is provided on the basis of a quotation after your request has been studied.<\/p><p class=\"rtejustify\" style=\"text-align: justify;\">Users must provide all information relating to the possible risks for handlers. The cost of analysis is given in the summary table.<\/p><h4>\u00a0<\/h4><h4 class=\"rtejustify\" style=\"text-align: justify;\"><span style=\"color: #003366;\"><strong>Use of equipment<\/strong><\/span><\/h4><p class=\"rtejustify\" style=\"text-align: justify;\">Users authorised for free access undertake to systematically fill in the \"user monitoring booklet\" provided and to report any problems encountered during use on the booklet and on GRR. In the event of a malfunction, they must contact the equipment manager. All users are responsible for leaving the equipment clean and tidy at the end of the session.<\/p><h4>\u00a0<\/h4><h4 class=\"rtejustify\" style=\"text-align: justify;\"><span style=\"color: #003366;\"><strong>Reservations<\/strong><\/span><\/h4><p class=\"rtejustify\" style=\"text-align: justify;\">Reservations for equipment can only be made through the <a href=\"https:\/\/grr.univ-tln.fr\/\" target=\"_blank\" rel=\"noopener\">Intranet site<\/a> or by PSOI-CN staff after validation of the analysis request. Users are notified by e-mail when a device is unavailable.<\/p><h4>\u00a0<\/h4><h4 class=\"rtejustify\" style=\"text-align: justify;\"><span style=\"color: #003366;\"><strong>Services<\/strong><\/span><\/h4><p class=\"rtejustify\" style=\"text-align: justify;\">For a service or collaboration, users (applicants) must request an appointment by e-mail or telephone with the Centre's managers in order to establish the costs and, if necessary, an estimate.<\/p><p class=\"rtejustify\" style=\"text-align: justify;\">The P\u00f4le can provide the following services:<\/p><ol><li class=\"rteindent1 rtejustify\" style=\"text-align: justify;\">A consultancy business to study the fluorescence of organic matter<\/li><li class=\"rteindent1 rtejustify\" style=\"text-align: justify;\">Support during acquisitions on different instruments and during analysis on PARAFAC or deconvolution on MATLAB<\/li><li class=\"rteindent1 rtejustify\" style=\"text-align: justify;\">Theoretical and practical training :<\/li><\/ol><ul><li style=\"list-style-type: none;\"><ul><li class=\"rteindent2 rtejustify\" style=\"text-align: justify;\">Fluorescence spectroscopy \"source separation and time resolution - from photon to source\" (40H)<\/li><li class=\"rteindent2 rtejustify\" style=\"text-align: justify;\">Laser risks and prevention \"Laser risks and prevention awareness training for anyone working with laser sources\" (4H).<\/li><\/ul><\/li><\/ul><h4 style=\"padding-left: 40px;\">\u00a0<\/h4><h4 class=\"rtejustify\" style=\"text-align: justify;\"><span style=\"color: #003366;\"><strong>Fluorescence spectroscopy analysis<\/strong><\/span><\/h4><ul class=\"rteindent1\" style=\"text-align: justify;\"><li class=\"rtejustify\">\u00a02D Fluorescence Spectrum Measurement<\/li><li class=\"rtejustify\">\u00a03D Fluorescence Spectrum Measurement<\/li><li class=\"rtejustify\">\u00a0Fluorescence quenching<\/li><li class=\"rtejustify\">\u00a0Fluorescence Emission Excitation Matrix (FEEM)<\/li><li class=\"rtejustify\">\u00a0Time-to-live measurements (TRLF) on liquid and solid samples with lifetimes from nanoseconds to microseconds.<\/li><li class=\"rtejustify\">\u00a0PARAFAC breakdown of the ProgMEEF and ProgTIME programmes developed in the laboratory by Roland Redon<\/li><li class=\"rtejustify\">\u00a0Measurement of absorption in the 200-800 nm wavelength range using UV-Visible Spectroscopy.<\/li><\/ul><h4 style=\"text-align: justify;\">\u00a0<\/h4><h4 class=\"rtejustify\" style=\"text-align: justify;\"><span style=\"color: #003366;\"><strong>Confocal UV, Visible and Biphotonic imaging analyses <\/strong><\/span><\/h4><ul class=\"rteindent1\" style=\"text-align: justify;\"><li class=\"rtejustify\">\u00a03D imaging.<\/li><li class=\"rtejustify\">\u00a0Spectral imaging.<\/li><li class=\"rtejustify\">\u00a0Fluorescence redistribution after photobleaching (FRAP, FLIP)<\/li><li class=\"rtejustify\">\u00a0Fluorescence Lifetime (FLIM)<\/li><li class=\"rtejustify\">\u00a0F\u00f6rster-type resonant energy transfer by FLIM<\/li><\/ul><h4 style=\"text-align: justify;\">\u00a0<\/h4><h4 class=\"rtejustify\" style=\"text-align: justify;\"><span style=\"color: #003366;\"><strong>Equipment <\/strong><\/span><\/h4><ul class=\"rteindent1\" style=\"text-align: justify;\"><li class=\"rtejustify\">\u00a0HITACHI F-7000<\/li><li class=\"rtejustify\">\u00a0HITACHI F-4500 with sample holder for automatic measurement of 16 samples<\/li><li class=\"rtejustify\">\u00a0SHIMADZU UV-1800<\/li><li class=\"rtejustify\">\u00a0Laser Spectra-Physics Quanta-Ray INDI Nd:YAG Wavelengths: 1064 nm, 532 nm, 355 nm and 266 nm -Pulse width: 3-5 ns - Frequency: 20 Hz<\/li><li class=\"rtejustify\">\u00a0EKSPLA picosecond Nd:YAG laser Wavelengths: 1064 nm, 532 nm, 355 nm and 266 nm - Pulse width: 20 ps - Frequency: 20 Hz<\/li><li class=\"rtejustify\">\u00a0Zeiss upright microscope LSM 880 Laser Visible 458, 488, 514, 561, 633 nm - LASER Ti:Sapphire Chameleon Ultra II from 680 to 1080 nm - HARMONIXX UV PULSED LINE 340 to 540 nm - FLIM-Becker system for lifetime measurement - Chamber with temperature regulation\/gas injection<\/li><li class=\"rtejustify\">TOC-V analyser - SHIMADZU<\/li><li class=\"rtejustify\">Total nitrogen analyser, NT. TNM-1 module - SHIMADZU<\/li><li class=\"rtejustify\">Elemental analyser CHNS, FLASH 2000 - ThermoScientific<\/li><\/ul><p>\u00a0<\/p><p class=\"rtejustify\" style=\"text-align: justify;\">Download the price list in pdf : <a href=\"https:\/\/nuage.osupytheas.fr\/s\/ziNHEQrPe4BCYcM\" target=\"_blank\" rel=\"noopener\">ICI<\/a>\u00a0<\/p><p class=\"rtejustify\" style=\"text-align: justify;\">Analysis request form : <a href=\"https:\/\/nuage.osupytheas.fr\/s\/FDGs7BdnLMH9Ysw\" target=\"_blank\" rel=\"noopener\">ICI<\/a>\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-0d694e1 e-con-full e-flex e-con e-parent\" data-id=\"0d694e1\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t<div class=\"elementor-element elementor-element-a4d9462 e-flex e-con-boxed e-con e-child\" data-id=\"a4d9462\" data-element_type=\"container\">\n\t\t\t\t\t<div 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