SPECTROM

Spectroscopy, Optics and Imaging - Carbon, Nitrogen

Le Pôle Spectroscopie, Optique et Imagerie – Carbone, Azote rassemble les compétences de l’équipe CEM dans l’analyse par spectroscopie de fluorescence (continue et résolue en temps) et d’absorbance, appliquées notamment à la caractérisation de la matière organique (MO) ou la détection de composés fluorescents, en laboratoire mais également on-site et in-situ. En complément à cette caractérisation de la MO, le pôle propose aussi des analyses de carbone et d’azote total dans les compartiments liquide et solide.

Le Pôle a acquis récemment un microscope Confocal/biphoton à balayage laser qui permet d’obtenir des images de grande résolution. Des coupes « optiques » d’une épaisseur d’environ 0.4µm sont obtenues à différents niveaux de l’échantillon qui permettent une reconstruction 3D de l’échantillon.

This microscope is also capable of providing fluorescence lifetime images (FLIM) from a few hundred picoseconds down to microseconds. A typical application of FLIM is FLIM - FRET (Förster Resonance Energy Transfer). FRET is a well-established technique for studying molecular interactions. The cluster will develop these F-techniques to study complexations between organic matter and emerging pollutants.

Complementary techniques are used to determine concentrations of dissolved organic and inorganic carbon (DOC and DIC) using a TOC-V analyser and total nitrogen (TN) using the appropriate module. A CHNS will provide an elemental analysis of the solid matrix (soil, sediment, etc.).

How it works

SPECTROM is located on the premises of the Institut Méditerranéen d'Océanologie (MIO), at the University of Toulon on 1^er floor and 2^th building R, Campus La Garde.

The referents are :

Christophe LE POUPON Tel: 04 94 14 25 02 - e-mail: lepoupon@univ-tln.fr

Houssam HAJJOUL Tel: 04 94 14 27 94 - e-mail: hajjoul@univ-tln.fr

Access conditions

The Centre is accessible to all MIO and the scientific community: academics, industrialists and clinicians with study needs in UV-Visible spectroscopy (continuous and time-resolved fluorescence, fluorescence quenching, in-situ monitoring, etc.) and confocal microscopy (biphoton, 3D imaging, FLIM, FRET, FRAP, etc.).

To gain access to the self-service equipment, new users must undergo training on the equipment required, provided by the Hub's staff. Without this training, no novice user will be allowed to use the equipment. Access to the laser rooms is reserved for people with laser level I.

The Centre also offers simple services based on a research project or complete services from A to Z, including data analysis and results. In this case, the service is provided on the basis of a quotation after your request has been studied.

Users must provide all information relating to the possible risks for handlers. The cost of analysis is given in the summary table.

Use of equipment

Les utilisateurs autorisés à l’accès libre s’engagent à remplir systématiquement le « cahier du suivi des utilisateurs » mis à leur disposition et de signaler sur le cahier et sur GRR les problèmes rencontrés lors de l’utilisation. En cas de dysfonctionnement, ils doivent contacter le responsable de l’équipement. Chaque utilisateur est responsable de l’état de propreté dans lequel il laisse l’appareil en fin de séance.

Reservations

Equipment can only be booked via the website:
Intranet : https://grr.univ-tln.fr/ or by PSOI-CN staff after validation of the analysis request. Users are notified by e-mail when a device is unavailable.

Services

For a service or collaboration, users (applicants) must request an appointment by e-mail or telephone with the Centre's managers in order to establish the costs and, if necessary, an estimate.

The Pôle can provide the following services:

A. A consultancy business to study the fluorescence of organic matter
B. Support during acquisitions on different instruments and during analysis on PARAFAC or deconvolution on MATLAB
C. Theoretical and practical training :

- Fluorescence spectroscopy "source separation and time resolution - from photon to source" (40H)
- Laser risks and prevention "Laser risks and prevention awareness training for anyone working with laser sources" (4H)

Fluorescence spectroscopy analysis

2D Fluorescence Spectrum Measurement
3D Fluorescence Spectrum Measurement
Fluorescence quenching
Fluorescence Emission Excitation Matrix (FEEM)
Time-to-live measurements (TRLF) on liquid and solid samples with lifetimes from nanoseconds to microseconds.
PARAFAC breakdown of the ProgMEEF and ProgTIME programmes developed in the laboratory by Roland Redon
Mesure de l’absorption dans le domaine de longueurs d’onde 200-800 nm par Spectroscopie UV-Visible.

Confocal UV, Visible and Biphotonic imaging analyses

3D imaging.
Spectral imaging.
Fluorescence redistribution after photobleaching (FRAP, FLIP)
Fluorescence Lifetime (FLIM)
Le transfert résonnant d’énergie de type Förster par FLIM

Equipment

HITACHI F-7000
HITACHI F-4500 with sample holder for automatic measurement of 16 samples
SHIMADZU UV-1800
Laser Spectra-Physics Quanta-Ray INDI Nd:YAG Wavelengths: 1064 nm, 532 nm, 355 nm and 266 nm -Pulse width: 3-5 ns - Frequency: 20 Hz
EKSPLA picosecond Nd:YAG laser Wavelengths: 1064 nm, 532 nm, 355 nm and 266 nm - Pulse width: 20 ps - Frequency: 20 Hz
Zeiss upright microscope LSM 880 Laser Visible 458, 488, 514, 561, 633 nm - LASER Ti:Sapphire Chameleon Ultra II from 680 to 1080 nm - HARMONIXX UV PULSED LINE 340 to 540 nm - FLIM-Becker system for lifetime measurement - Chamber with temperature regulation/gas injection
TOC-V analyser - SHIMADZU
Total nitrogen analyser, NT. TNM-1 module - SHIMADZU
Elemental analyser CHNS, FLASH 2000 - ThermoScientific

Download the price list in pdf : ICI (clickable link)

Fiche de demande d’analyses : ICI (clickable link)

Visit SPECTROM in pictures

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Mediterranean Institute of Oceanology